Mouse TNF alpha DIY ELISA Kit

The Assay Genie Mouse TNF alpha DIY ELISA Kit enables quantitative measurement of Tumor Necrosis Factor alpha, a key pro-inflammatory cytokine implicated in inflammation, sepsis, and autoimmune disease, in samples collected from mouse experimental systems. The DIY workflow requires the researcher to coat the microplate with the supplied capture antibody, providing flexibility to run batches from one to ten plates as needed.

Reported performance specifications include a detection range of 15.6 to 1000 pg/mL, sensitivity below 10 pg/mL, and a 100 uL sample volume per well. The assay uses a TMB substrate for colorimetric readout and supplies lyophilized recombinant TNF-alpha standards for reliable curve fitting. Unopened reagents should be refrigerated and protected from light; once reconstituted, aliquot and store according to the manual to limit freeze-thaw cycles.

Common research applications include characterizing cytokine release in cell culture supernatants, profiling inflammatory responses in animal disease models, and screening pharmacologic compounds for immunomodulatory activity. For research use only.