Mouse TLR-2 (Toll-like Receptor 2) ELISA Kit (MOES01698)

TLR-2 (Toll-like Receptor 2) is a pattern recognition receptor of the innate immune system that detects bacterial lipoproteins and peptidoglycan, triggering MyD88-dependent NF-κB inflammatory signaling. This sandwich ELISA from Assay Genie is configured to quantify mouse TLR-2 protein in serum, plasma, and cell culture supernatant samples using a 96-well microplate format suited to batch processing of murine experimental cohorts.

Researchers apply the kit to investigate bacterial infection responses, sepsis models, periodontal inflammation, autoimmune disease mechanisms, and TLR-2 agonist vaccine adjuvant studies. The antibody pair is specific to mouse TLR-2 with no significant cross-reactivity observed against rat or human samples under standard assay conditions. Each kit contains a pre-coated capture plate, biotinylated detection antibody, protein standards, streptavidin-HRP conjugate, and TMB substrate, providing sufficient reagents for 96 individual assays.

Standard protocol involves a 100 µL sample volume per well, approximately 2.5 hours of total incubation at 37°C, and a colorimetric readout compatible with standard laboratory plate readers. Recommended storage is refrigerated between uses, with shelf life extending several months when components are handled per the included manual.