Mouse TJP1 (Tight Junction Protein 1) ELISA Kit (MOES01563)

This Sandwich ELISA Kit from Assay Genie is designed for the precise measurement of tight junction protein 1 (TJP1) levels in mouse serum, plasma, and cell culture supernatants. The assay uses a colorimetric Sandwich-ELISA principle: the microplate is pre-coated with an antibody specific to the target, and a biotinylated detection antibody followed by an Avidin-Horseradish Peroxidase (HRP) conjugate is used to generate a colour change that is read at 450 nm ± 2 nm.

Performance characteristics reported by the manufacturer include a sensitivity of 46.88 pg/mL, a detection range of 78.13-5000 pg/mL, a recovery of 80%-120%, and an assay time of 3 h 30 min. The recommended sample volume is 100 μL, the kit is calibrated to UniProt ID P39447, and specificity testing indicated no significant cross-reactivity or interference between Mouse TJP1 and analogues.

Tight junction protein 1 is a key component of tight junctions, critical for maintaining the integrity of epithelial and endothelial cell barriers. Dysregulation of TJP1 has been linked to inflammatory bowel disease, liver diseases, and cancer. The kit is intended for storage at 2-8°C and is targeted at research in Cancer, Tags and Cell Markers.