Mouse Tissue factor/Coagulation Factor III ELISA Kit

This sandwich ELISA kit from Assay Genie is designed for the quantitative measurement of tissue factor (coagulation factor III) in mouse samples. It uses a colorimetric, double-antibody sandwich detection format and is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, with storage at 2–8°C for 12 months.

Tissue factor, also known as coagulation factor III, is described as a critical protein in the initiation of the coagulation cascade, playing a central role in blood clotting and thrombosis and serving as a biomarker of interest in thrombotic and cardiovascular disease research. Associated identifiers listed by the manufacturer include UniProt P20352 and Gene ID 14066, and the research area is given as cardiovascular. The kit reports a sensitivity of 3.75 pg/mL and a working range of 6.25–400 pg/mL.

Linearity across 1:2 to 1:8 dilutions is reported as 88–97% / 83–99% / 80–98% for serum, 86–98% / 90–98% / 82–97% for EDTA plasma, and 91–104% / 86–101% / 82–100% for heparin plasma. Spike-and-recovery averages are 94% for serum, 94% for EDTA plasma, and 93% for heparin plasma.