Mouse TIMP-4 ELISA Kit

From Assay Genie, this is a sandwich ELISA, double antibody assay designed for the precise measurement of tissue inhibitor of metalloproteinase 4 (TIMP-4) in mouse samples. The kit is reactive with mouse serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, and uses a colorimetric detection method with absorbance read at 450 nm. The associated Uniprot identifier is Q9JHB3.

The assay reports a sensitivity of 37.5 pg/mL and an assay range of 62.5-4000 pg/mL. Linearity at dilutions of 1:2, 1:4, and 1:8 was 88-103%, 84-98%, and 88-100% for serum (n=5); 88-102%, 82-101%, and 80-99% for EDTA plasma (n=5); and 95-105%, 88-99%, and 81-97% for heparin plasma (n=5). Recovery ranges were 90-102% for serum (average 95%), 87-95% for EDTA plasma (average 90%), and 91-100% for heparin plasma (average 95%). Storage is at 2-8°C for 12 months.

TIMP-4 is described as a key regulator of extracellular matrix remodeling involved in tissue homeostasis and development, with dysregulation linked to cancer, cardiovascular disorders, and fibrotic conditions. The assay workflow uses a biotin-labeled detection antibody, HRP-streptavidin conjugate, and TMB substrate, with primary incubation at 37°C for 90 minutes.