Mouse Thyrotropin subunit beta/TSHB ELISA Kit

A competitive enzyme-linked immunosorbent assay (ELISA) from Assay Genie designed for the sensitive and accurate quantification of mouse Thyrotropin subunit beta (TSHB). TSHB is an important subunit of thyrotropin (TSH), the hormone that regulates thyroid gland function and plays a central role in metabolism. Abnormal TSH levels are linked to thyroid disorders such as hypothyroidism and hyperthyroidism, making this kit a valuable resource for endocrinology research and for studying thyroid function, hormone regulation, and related disorders in mouse models.

The kit uses a colorimetric competitive ELISA format with antigen-coated wells and is reactive with mouse serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Performance specifications include a sensitivity of 0.094 uIU/mL and a detection range of 0.156–10 uIU/mL. Components are intended to be stored sealed at 2–8°C and should not be cryopreserved. The target protein is catalogued under UniProt P12656.

Assay performance is supported by linearity data generated at 1:2, 1:4, and 1:8 dilutions across serum, EDTA plasma, and heparin plasma, and by recovery data showing serum at 93–100% (average 95%), EDTA plasma at 86–101% (average 83%), and heparin plasma at 81–98% (average 96%).