Mouse Thrombospondin-4 (Thbs4) ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is designed for the quantitative measurement of Mouse Thrombospondin-4 (THBS4), also referred to as Tsp4, using a colorimetric detection method. It is reactive with mouse samples and validated for use with serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids. The kit is intended for research use only and is suited to cardiovascular research applications.

Reported performance specifications include a detection range of 78-5000pg/mL and a sensitivity of 39.7pg/mL. Intra-assay CV is 3.9% and inter-assay CV is 6.1%. Recovery data indicate averages of 80% (range 80-85%) for serum and 81% (range 80-87%) for plasma, with linearity demonstrated across 1:2 to 1:16 serial dilutions in serum, EDTA plasma, and heparin plasma. The assay recognises natural and recombinant mouse Thrombospondin-4, corresponding to UniProt entry Q9Z1T2 (NCBI Gene ID 21828), with a molecular weight of 106,366 Da reported for the protein.

THBS4 functions as an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. It is involved in cellular proliferation, migration, adhesion and attachment, inflammatory response to CNS injury, regulation of vascular inflammation, and adaptive responses of the heart to pressure overload, including myocardial function and remodeling. The protein localises to the endoplasmic reticulum, sarcoplasmic reticulum, and secreted extracellular compartments, including the extracellular matrix, where it contributes to cardioprotective and adaptive ECM remodeling.