Mouse Thrombopoietin (Thpo) ELISA Kit

Assay Genie offers a Sandwich ELISA Kit for the quantitative detection of Mouse Thrombopoietin (Thpo), with reactivity against mouse samples. The kit is intended for research use only and is suitable for use with serum, plasma, tissue homogenates, cell culture supernates and other biological fluids, using a colorimetric detection method.

Manufacturer-reported performance characteristics include a detection range of 31.2-2000pg/ml, a sensitivity of 15.83pg/mL, an intra-assay CV of 6.4%, and an inter-assay CV of 8.6%. Linearity has been assessed across serial dilutions (1:2, 1:4, 1:8, 1:16) in serum (N=5) at 90-100%, 89-98%, 112-122% and 107-117%; in EDTA plasma (N=5) at 104-113%, 106-116%, 106-118% and 102-114%; and in heparin plasma (N=5) at 103-115%, 91-101%, 102-112% and 104-113%. Recovery testing returned an average of 83% (range 80-89%) for serum and 85% (range 80-91%) for plasma. Specificity is described for natural and recombinant mouse Thrombopoietin.

Thrombopoietin is described as a lineage-specific cytokine that affects the proliferation and maturation of megakaryocytes from their committed progenitor cells, acting at a late stage of megakaryocyte development and acting as a major physiological regulator of circulating platelets. The protein is secreted and the associated UniProt entry is P40226. Listed synonyms include C-mpl ligand, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, Myeloproliferative leukemia virus oncogene ligand, ML, and MGDF. According to third-party supplier information, the kit should be stored at -20°C and is shipped at +4°C.