Mouse Thrombin-antithrombin complex/TAT ELISA Kit

A colorimetric Sandwich ELISA from Assay Genie designed for the quantitative measurement of thrombin-antithrombin complex (TAT) in mouse serum, plasma and other biological fluids. The assay is intended as a research tool for studies of coagulation, hemostasis and inflammation in mouse models. The thrombin-antithrombin complex is a marker of coagulation, reflecting the balance between thrombin generation and its inhibition by antithrombin, and its dysregulation has been implicated in pathological conditions such as thrombosis, disseminated intravascular coagulation and sepsis.

The Sandwich-ELISA procedure uses wells pre-coated with a target-specific antibody. Standards or samples, a biotinylated detection antibody and an Avidin-Horseradish Peroxidase (HRP) conjugate are added sequentially with washing steps. A substrate solution is then added, producing a blue colour that turns yellow upon addition of stop solution. Optical density is read at 450 nm ± 2 nm and is directly proportional to target concentration. The total assay time is 3 h 30 min. The kit is supplied with a micro ELISA plate, reference standard, concentrated biotinylated detection antibody, concentrated HRP conjugate, reference standard and sample diluent, detection antibody diluent, HRP conjugate diluent, concentrated wash buffer, substrate reagent, stop solution, plate sealer, technical manual and certificate of analysis.

Reported performance includes a sensitivity of 9.38 pg/mL, a detection range of 15.63-1000 pg/mL, and recovery of 80%-120%. The kit recognizes Mouse TAT, with no significant cross-reactivity or interference between Mouse TAT and analogues observed. Recommended storage is 2-8°C. Research areas include cancer, cell biology, cardiovascular and signal transduction.