Mouse Thioredoxin (Txn) ELISA Kit

A high-quality sandwich ELISA kit from Assay Genie designed for the quantitative detection of mouse thioredoxin (Txn), an essential antioxidant protein that plays a critical role in redox regulation and cell signalling pathways. The assay is reactive with mouse samples and is suited to research applications in oxidative stress, cell biology and cancer research, where thioredoxin serves as a biomarker for studying DNA synthesis, cell growth and apoptosis.

Performance characteristics include a detection range of 1.56–100 ng/mL, a sensitivity of 0.78 ng/mL, an intra-assay CV of 5.8% and an inter-assay CV of 8.3%. Linearity has been validated across dilutions from 1:2 to 1:16 in serum (80–116%), EDTA plasma (91–124%) and heparin plasma (97–112%). Recovery averages 106% for serum (range 100–112%) and 108% for plasma (range 102–114%). The assay specifically recognises natural and recombinant mouse thioredoxin and is for research use only.

Accepted sample types include serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids. Known synonyms for the target are ATL-derived factor, ADF, Trx and Txn1 (UniProt accession P10639). The associated function notes that ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55), and the listed research area is epigenetics.