Mouse TFPI/Tissue factor pathway inhibitor ELISA Kit

Assay Genie's Mouse TFPI/Tissue factor pathway inhibitor ELISA Kit is a colorimetric sandwich ELISA designed for the accurate detection of TFPI levels in mouse samples. TFPI is a key regulator of the blood coagulation pathway that inhibits the tissue factor pathway, and its dysregulation has been linked to thrombosis, sepsis, and inflammatory disorders. The kit has a sensitivity of 46.875 pg/mL with a working range of 78.125-5000 pg/mL, and is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

Assay performance is supported by linearity and recovery data across serum, EDTA plasma, and heparin plasma matrices, with recovery averages of 95%, 93%, and 92% respectively. The double-antibody sandwich format uses a biotin-labeled detection antibody and HRP-streptavidin conjugate, with color development read at 450 nm after TMB substrate addition. The kit is intended for cardiovascular and hemostasis research, including investigations into coagulation and inflammation, and is stored at 2-8°C for 12 months.