Mouse TFPI (Tissue Factor Pathway Inhibitor) ELISA Kit (MOES01564)

This quantitative immunoassay from Assay Genie measures mouse Tissue Factor Pathway Inhibitor, a Kunitz-type serine protease inhibitor that regulates the extrinsic coagulation cascade by blocking factor VIIa–tissue factor complexes. Researchers use it to investigate thrombotic and hemostatic mechanisms in murine models, including studies of atherosclerosis, sepsis-related coagulopathy, and gene-knockout phenotypes affecting TFPI expression.

Each kit provides a pre-coated microplate along with all reagents needed to generate a standard curve and run unknown samples in duplicate. Serum collected from mouse models and EDTA-treated plasma specimens are the most commonly processed matrices, though cell culture supernatants and tissue homogenates can also be evaluated when validated by the user. Capture antibodies specific for mouse TFPI are immobilized on the plate, and an enzyme-conjugated secondary antibody produces a measurable color change proportional to antigen concentration in each well.

Intended for research use only, the assay supports both acute and longitudinal study designs, allowing investigators to track circulating TFPI levels across treatment groups or disease progression. The complete workflow of coating, incubation, washing, and signal development is compatible with standard plate readers equipped with a visible-light filter, making it accessible to most laboratory settings without specialized equipment.