Mouse TFF2 (Trefoil Factor 2) CLIA Kit (MOES00579)

The Mouse TFF2 (Trefoil Factor 2) CLIA Kit from Assay Genie is a chemiluminescence-based sandwich immunoassay that quantitatively detects murine trefoil factor 2 in serum, plasma, and other biological fluids. Trefoil factor 2 is a small secreted peptide predominantly expressed in the gastric mucosa and gastrointestinal tract, where it contributes to mucosal repair, epithelial restitution, and protection against injury. Researchers commonly apply this kit in studies of gastrointestinal inflammation, gastric ulcer healing, mucosal immunology, and gastric cancer biomarker work in mouse models.

The assay is supplied as a 96-well microplate pre-coated with capture antibody, together with standards, detection reagents, and buffer solutions needed to complete the run. The chemiluminescent readout provides high analytical sensitivity, allowing the kit to detect low-abundance TFF2 in dilute samples. Typical protocols use 100 µl of sample per well, with total assay time of approximately 3.5 hours from plate setup to final measurement.

For optimal stability, unopened kit components should be stored at 4°C, while reconstituted standards and working solutions should be kept at -20°C in single-use aliquots to limit freeze-thaw cycles. This product is intended for research use only and is not validated for diagnostic or clinical applications.