Mouse Testosterone ELISA Kit

The Mouse Testosterone ELISA Kit from Assay Genie is a competitive immunoassay for the quantitative determination of testosterone in mouse serum and plasma samples. Testosterone, the primary male androgen, plays a central role in reproductive biology, sexual development, and metabolic regulation, and accurate quantification in rodent models supports studies in endocrinology, reproduction, toxicology, and behavioral neuroscience.

The kit is supplied as a complete set with a pre-coated 96-well microplate, calibration standards, detection antibody, wash buffer, and substrate solution. The colorimetric endpoint is read at 450 nm on a standard microplate reader, and the signal decreases as testosterone concentration rises, enabling construction of a standard curve for interpolation of unknown samples. Performance characteristics include a low detection limit, intra- and inter-assay reproducibility reported as %CV, and a dynamic range spanning physiological and pharmacological testosterone levels in mouse specimens. Typical sample volume per well is 10 µL of serum or plasma.

For reliable performance, serum and plasma samples should be collected under standard conditions; the kit and reagents should be stored refrigerated at 2-8°C between uses, protected from light, and equilibrated to room temperature before the protocol. Aliquot samples to limit freeze-thaw cycles and run the assay in vitro according to the supplied insert. This product is intended for research use only and is compatible with common automated and manual plate readers.