Mouse TAT (Thrombin-Antithrombin Complex) ELISA Kit (MOES01545)

A colorimetric sandwich ELISA for the detection of mouse thrombin-antithrombin complex (TAT), supplied by Assay Genie. The assay is designed to measure TAT in mouse serum, plasma, cell culture media and other biological fluids, and is intended for research into coagulation and hemostasis, thrombosis and inflammation in mouse models.

The manufacturer reports an assay time of 3 h 30 min and a sample volume of 100 μL, with a sensitivity of 9.38 pg/mL and a detection range of 15.63-1000 pg/mL. The stated recovery specification is 80%-120%. Sample-specific recovery results list EDTA Plasma (n=8) at 88-99% range with 93% average recovery, and Cell Culture Media (n=8) at 85-98% range with 91% average recovery. Reported coefficient of variation values are 6.61%, 4.65% and 4.56% for intra-assay precision and 6.62%, 6.75% and 8.52% for inter-assay precision.

Kit components listed by the manufacturer include a reference standard, concentrated biotinylated detection antibody, concentrated HRP conjugate, reference standard and sample diluent, detection antibody diluent, HRP conjugate diluent, concentrated wash buffer, substrate reagent, stop solution, plate sealers, a technical manual and a certificate of analysis. Kit storage is given as 2-8°C, with listed components stored at 2-8°C or -20°C. The manufacturer states that the kit recognizes mouse TAT with no significant cross-reactivity or interference observed between mouse TAT and analogues, and lists the research areas as cancer, cell biology, cardiovascular and signal transduction.