Mouse TARS (Threonyl tRNA Synthetase, cytoplasmic) ELISA Kit (MOES01809)

Building on that foundation, this 96-well kit from Assay Genie uses a quantitative two-antibody capture format in which a biotin-conjugated detection reagent and a Streptavidin-HRP conjugate produce the visible signal measured at 450 nm. The pre-coated capture antibody recognizes native TARS rather than recombinant protein, and no significant cross-reactivity with homologous mouse aminoacyl-tRNA synthetases has been observed. Recommended plasma handling uses EDTA as the anticoagulant, with centrifugation at 1,000 x g for 15 minutes at 2-8 °C within 30 minutes of collection; serum is clotted at room temperature for 2 hours or refrigerated overnight before a similar 1,000 x g spin. Each well receives 100 µL of standard or sample followed by 100 µL of biotinylated antibody working solution, with incubations carried out at 37 °C under the supplied plate sealer. The kit supplies a lyophilized standard curve, a 25× wash buffer concentrate, TMB substrate, and stop solution configured for the 96-assay microplate, and all components are intended for research use only in studies of class-II aminoacyl-tRNA synthetase biology, autoantibody profiling in inflammatory myopathy models, and translational control research.