Mouse Synaptosomal Associated Protein 25kDa (SNAP25) ELISA Kit

The SNAP25 ELISA Kit from Assay Genie enables quantitative measurement of mouse Synaptosomal-Associated Protein 25 in research samples using an immunoassay workflow. SNAP25 is a 25 kDa presynaptic SNARE complex component that mediates calcium-triggered vesicle fusion and neurotransmitter release at central synapses, and it is frequently profiled in neuroscience research on synaptic function, neurodegenerative disease models, and pancreatic beta-cell exocytosis studies.

Each kit supplies a pre-coated microplate and reagent set sufficient for 96 assays. Standards and unknown samples are loaded into wells, then incubated with a biotinylated detection antibody and streptavidin-HRP conjugate; colorimetric absorbance readings are converted to protein concentration using the supplied standard curve. Mouse reactivity makes the kit appropriate for studies using rodent brain tissue homogenates and primary neuron or neuroblastoma cell lysates.

Kit components should be stored refrigerated upon arrival to maintain antibody binding activity and enzyme conjugate stability. The package insert provides a step-by-step protocol, recommended plate layouts, and representative standard curve data. This kit is intended for research use only and is not cleared for diagnostic applications. Researchers investigating synaptic vesicle cycling, neuronal development, or botulinum neurotoxin substrate cleavage will find this a focused reagent for monitoring SNAP25 abundance in experimental samples.