Mouse Synaptophysin ELISA Kit

Manufactured by Assay Genie, this ELISA kit is designed for the sensitive detection of synaptophysin levels in mouse samples. It employs a sandwich ELISA format with a double antibody approach and accepts serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The manufacturer describes synaptophysin as a key protein found in neuronal synaptic vesicles, where it plays a vital role in neurotransmission and synaptic plasticity, and notes that studying its levels may provide insights into conditions such as Alzheimer's disease, Parkinson's disease, and schizophrenia.

The kit has a stated sensitivity of 0.375 ng/mL and a range of 0.625-40 ng/mL. Storage conditions are 2-8°C for 12 months. Manufacturer-reported recovery data show serum averaging 96% (range 86-102%), EDTA plasma averaging 93% (range 86-101%), and heparin plasma averaging 93% (range 86-100%). Linearity across dilutions 1:2, 1:4, and 1:8 is reported for serum (87-95%, 82-94%, 90-105%), EDTA plasma (80-90%, 86-101%, 83-101%), and heparin plasma (91-105%, 85-96%, 82-98%). The assay procedure involves loading samples and standards on a pre-coated plate, incubating at 37°C for 90 minutes, adding biotin-labeled detection antibody with a 60-minute incubation, adding HRP-Streptavidin (SABC) for 30 minutes, developing with TMB substrate in the dark for 10-20 minutes, then adding stop solution and reading absorbance at 450 nm.

Associated identifiers include UniProt ID Q62277 and Gene ID GeneID 20977. Listed synonyms are SYP, Major synaptic vesicle protein p38, MRX, MRXSYP, and synaptophysin. Shipping is reported at +4°C.