Mouse Surfactant Associated Protein A (SPA) ELISA Kit

Quantitative profiling of pulmonary collectins in murine respiratory models is supported by this microplate-based colorimetric ELISA from Assay Genie, configured to quantify Surfactant Associated Protein A in lung-derived and peripheral specimens. SP-A, the dominant collectin secreted by alveolar type II cells and Clara cells, serves as a biomarker for alveolar epithelial integrity, surfactant pool homeostasis, and innate pulmonary immunity in mouse studies of acute lung injury, neonatal respiratory distress, cystic fibrosis, COPD, bleomycin-induced fibrosis, smoke inhalation, and bacterial or viral pulmonary infection.

Compatible specimen types include bronchoalveolar lavage fluid, lung tissue homogenates, cell culture supernatants, and additional matrices harvested from murine studies. The kit supplies pre-coated plate strips, biotinylated detection antibody, recombinant protein standard, streptavidin-HRP conjugate, wash buffer concentrate, chromogenic substrate, and stop solution. Store kit components refrigerated upon arrival; aliquot standards and detection antibody for frozen storage to limit repeated freeze-thaw exposure across multi-week experiments.

Each lot is validated by Assay Genie for intra-assay and inter-assay precision. Researchers tracking SP-A dynamics alongside CC16, SP-D, IL-6, and BAL cytokine panels rely on this reagent pair for longitudinal phenotyping, transgenic comparison, and therapeutic intervention screening. The microplate layout accommodates a full standard curve plus quality-control replicates, supporting cohort-scale biomarker measurement in pulmonary research workflows.