Mouse Suppressors Of Cytokine Signaling 2 (SOCS2) ELISA Kit

Suppressors of Cytokine Signaling 2 (SOCS2) is a key intracellular regulator of the JAK-STAT signaling pathway, controlling the magnitude of cytokine and growth factor responses in immune and endocrine tissues. Researchers in immunology, inflammation biology, growth hormone research, and cancer pathway studies commonly quantify SOCS2 protein levels to assess negative feedback regulation of cytokine-driven signaling cascades.

This Mouse SOCS2 ELISA Kit from Assay Genie provides a quantitative immunoassay for measuring SOCS2 protein concentrations in mouse tissue homogenates and cell lysates, with results read on a standard microplate reader. The kit uses a matched-antibody-pair configuration with a colorimetric substrate and is supplied as a complete reagent set containing a pre-coated microplate, biotinylated detection antibody, streptavidin-HRP conjugate, lyophilized recombinant SOCS2 standards, wash buffer, substrate solution, and stop reagent - sufficient for 96 individual determinations.

Sample volume requirements are 100 uL per well, making the assay compatible with limited research specimens. The workflow involves sequential incubations totaling approximately 3-4 hours, suitable for batch processing of multiple mouse tissue samples. Unopened kits should be refrigerated and protected from light; reconstituted standards and opened reagents should be stored according to the instructions included in the product insert.

Intended for research use only, not for diagnostic or clinical applications. Mouse SOCS2 measurements obtained from this assay can be correlated with downstream STAT phosphorylation status, growth hormone sensitivity, and inflammatory cytokine responses across different mouse model systems.