Mouse Succinate Dehydrogenase Complex Subunit D (SDHD) ELISA Kit

The Mouse SDHD ELISA Kit from Assay Genie quantifies succinate dehydrogenase subunit D, a membrane-anchored iron-sulfur protein of mitochondrial respiratory Complex II that couples succinate oxidation to ubiquinone reduction in the TCA cycle. Researchers apply it to studies of Complex II assembly, mitochondrial bioenergetics, and tumor biology, including hereditary paraganglioma-pheochromocytoma syndromes linked to SDHD germline mutations, gastrointestinal stromal tumors, and hypoxia-driven metabolic reprogramming. Each microplate provides pre-coated wells, lyophilized recombinant standards, biotinylated detection antibody, streptavidin-HRP conjugate, and TMB substrate. Storage: keep sealed kit refrigerated at 4 degrees C and aliquot reconstituted standards for freezing below -20 degrees C, avoiding more than three freeze-thaw cycles. The procedure tolerates crude tissue homogenates prepared in non-denaturing lysis buffer, with a recommended sample volume of 50 uL per well. Results support investigations of mitochondrial respiratory chain integrity, Krebs cycle flux, and succinate accumulation in metabolic disease models.