Mouse SREBP (Sterol Regulatory Element Binding Protein) ELISA Kit (MOES01504)

The Mouse SREBP ELISA from Assay Genie offers quantitative measurement of Sterol Regulatory Element Binding Protein in mouse-derived research samples. SREBPs function as basic-helix-loop-helix-leucine zipper transcription factors that govern cholesterol biosynthesis, fatty acid synthesis, and triglyceride metabolism, making them key markers in studies of hepatic steatosis, NAFLD progression, dyslipidemia, and metabolic syndrome.

Each kit ships with a microplate pre-coated with capture antibody, lyophilized standards, detection reagent, and buffers sufficient for 96 determinations. The matched antibody pair recognizes epitopes on the mouse SREBP protein, allowing researchers to track activation of the lipogenic transcriptional program in response to dietary, genetic, or pharmacological interventions. Samples typically include tissue homogenates from liver or adipose, cultured cell lysates, or serum prepared under chilled conditions to preserve transcription factor stability.

The plate-based format supports simultaneous analysis of multiple experimental conditions and time points, with absorbance readout providing reproducible quantification against a standard curve. Researchers commonly pair this assay with qPCR analysis of downstream lipogenic genes such as FAS and ACC, or with western blot confirmation of precursor and nuclear SREBP isoforms. Store reagents refrigerated between uses and protect detection antibody from light to maintain activity.