Mouse SRD5A1/3-oxo-5-alpha-steroid 4-dehydrogenase 1 ELISA Kit

From Assay Genie, this Sandwich ELISA kit enables the in vitro quantitative determination of Mouse Srd5a1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1) in serum, plasma, and other biological fluids. The target is also referenced by the aliases SR type 1, Steroid 5-alpha-reductase 1, and S5AR 1, and the assay is reactive with mouse samples.

The kit reports a sensitivity of 0.094ng/ml and an assay range of 0.156-10ng/ml. Intra-assay precision is given as CV <8% and inter-assay precision as CV <10%. Reagents should be stored at 4°C for 6 months, and the product is labelled for research use only.

Spike-and-recovery performance was assessed across serum, EDTA plasma, and UFH plasma matrices, with averages of 91% (range 86-97%), 99% (range 87-105%), and 93% (range 87-104%), respectively. Linearity was evaluated by serial dilution of spiked samples in the same matrices. According to UniProt entry Q68FF9, Srd5a1 converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids, playing a central role in sexual differentiation and androgen physiology; the protein has a molecular weight of 29,344 Da.