Mouse Sphingosine 1 Phosphate Receptor 1 (S1PR1) ELISA Kit

This Assay Genie Sandwich ELISA Kit enables quantitative measurement of mouse Sphingosine 1-Phosphate Receptor 1 (S1PR1), also known as EDG-1, a G-protein-coupled receptor central to sphingolipid signaling in immune cell trafficking and vascular endothelial homeostasis. The microplate-based assay uses a mouse-specific capture antibody and is validated for tissue homogenates and cell lysates, supporting research into lymphocyte egress from lymphoid organs, endothelial barrier integrity, and pathways modulated by S1PR1 ligands such as sphingosine 1-phosphate and related therapeutics including fingolimod. Each kit contains a pre-coated 96-well plate, lyophilized protein standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, and substrate reagents sufficient for 96 assays. Researchers studying GPCR signaling, multiple sclerosis model systems, or vascular inflammation will find this quantitative assay useful for measuring receptor expression and regulation in cell culture and in vivo samples. Refrigerated storage of all reagents is recommended and the components should be protected from light to preserve activity, and the kit is intended for research use only.