Mouse Sphingosine 1 Phosphate Lyase 1 (SGPL1) ELISA Kit

Quantitative measurement of murine Sphingosine 1 Phosphate Lyase 1 (SGPL1) is the focus of this assay from Assay Genie, supporting research in sphingolipid metabolism, immune-cell trafficking, and nephrotic-syndrome models. SGPL1 is the terminal enzyme of the sphingolipid catabolic cascade, cleaving sphingosine-1-phosphate (S1P) into hexadecenal and phosphoethanolamine within the endoplasmic reticulum. Because intracellular S1P gradients regulate lymphocyte egress, endothelial barrier function, and inflammatory tone, tracking SGPL1 abundance in tissue homogenates helps connect genotype to phenotype in knockout and disease-model studies. The 96 assays configuration supplies a pre-coated microplate, biotinylated detection reagent, streptavidin-HRP conjugate, wash buffer, chromogenic substrate, and recombinant murine SGPL1 standards sufficient to generate a full standard curve. Each run uses a small sample volume per well, and absorbance readings on a standard plate reader yield interpolated concentrations across multiple replicates. Researchers routinely pair this assay with qPCR or western blot to corroborate transcription- and protein-level changes in liver, kidney, thymus, and spleen lysates. Unused reagents should be refrigerated at 4 °C, with the standard stock aliquoted and frozen to limit freeze-thaw cycles and preserve linearity across batches.