Mouse Sodium/potassium-transporting ATPase subunit alpha-3 (Atp1a3) ELISA Kit

A sandwich ELISA kit from Assay Genie designed for the quantitative measurement of mouse Sodium/potassium-transporting ATPase subunit alpha-3 (Atp1a3) in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids. The assay uses a colorimetric detection method and shows specificity for natural and recombinant mouse ATP1A3.

Reported performance characteristics include a detection range of 78-5000 pg/mL, a sensitivity of 41.7 pg/mL, an intra-assay CV of 6.2%, and an inter-assay CV of 8.1%. Linearity has been evaluated across serum, EDTA plasma, and heparin plasma at serial dilutions. Recovery averages 87% (range 81-93%) in serum and 89% (range 83-95%) in plasma. ATP1A3 functions as the catalytic component of the Na+/K+ ATPase, hydrolyzing ATP to drive the exchange of sodium and potassium ions across the plasma membrane. The protein is a multi-pass membrane protein located in the cell membrane and belongs to the P-type cation transport ATPase family (UniProt: Q6PIC6).

Kit components are stored according to specification, with most reagents kept at -20°C and select buffers at 4°C. The product ships at +4°C and is intended for research use only.