Mouse Sodium-dependent serotonin transporter (Slc6a4) ELISA Kit

This Sandwich ELISA kit from Assay Genie is designed for the quantitative determination of mouse sodium-dependent serotonin transporter (SLC6A4) in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. A colorimetric detection method supports reproducible measurement, and the kit is supplied for research use only.

SLC6A4 is a key regulator of serotonergic signaling, mediating reuptake of serotonin from the synaptic cleft back into the pre-synaptic terminal in a sodium-dependent manner, with roles in mood regulation, behaviour and neurotransmission in mouse models. Dysregulation of SLC6A4 has been linked to psychiatric conditions including depression, anxiety and obsessive-compulsive disorder, making it a valuable biomarker for neuroscience research. The kit is specific for natural and recombinant mouse SLC6A4 and recognises the target as a multi-pass membrane protein located at the cell membrane, endomembrane system and endosome membrane. Reported synonyms for the target include 5HT transporter, 5HTT, SERT, Htt, Sert and solute carrier family 6 member 4.

Manufacturer-reported performance characteristics include a detection range of 15.6-1000pg/mL, a sensitivity of 8.02pg/mL, an intra-assay CV of 4.5% and an inter-assay CV of 7.5%. Recovery in serum averaged 103% (range 97-109%) and in plasma averaged 105% (range 99-111%), with linearity demonstrated across serial dilutions for serum, EDTA plasma and heparin plasma samples.