Mouse SNCa (Synuclein, Alpha) CLIA Kit (MOES00545)

Assay Genie's CLIA platform provides broader dynamic range and lower background than colorimetric ELISA, which is especially valuable when quantifying the relatively low circulating concentrations of alpha-synuclein in mouse blood. The chemiluminescent signal is typically captured on a luminometer equipped with 96-well plate reading capability; readings remain stable for an extended window after substrate addition, giving flexibility in plate-reader scheduling. For optimal reproducibility, serum and plasma samples should be collected on ice, centrifuged at 1000 x g for 10 minutes, and aliquoted into single-use vials before storage at -80 °C to minimize freeze-thaw cycles.

The assay shows minimal cross-reactivity with beta-synuclein and gamma-synuclein isoforms, supporting interpretation in studies that distinguish alpha-synuclein pathology from broader synuclein family changes. Researchers commonly apply this kit to characterize SNCA transgenic lines such as A53T and to monitor alpha-synuclein levels in CSF, brain homogenates, and conditioned media from primary neuronal cultures. Each lot is validated for intra-assay CV below 10% and inter-assay CV below 15%, and the kit ships on cold packs with a shelf life of up to 12 months when stored at 2-8 °C.