Mouse Smad7 ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is designed for the precise measurement of SMAD7 levels in mouse samples, including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Detection is colorimetric, using a sandwich ELISA, double antibody format with read-out by absorbance at 450 nm after TMB substrate development.

SMAD7 is described as a key regulator in the TGF-Beta signaling pathway, playing a critical role in cell differentiation, growth, and apoptosis. Dysregulation of SMAD7 has been implicated in diseases such as cancer, fibrosis, and inflammatory disorders, making it a valuable biomarker for research into biological processes and disease states. The kit reports a sensitivity of 0.188 ng/mL and an assay range of 0.313-20 ng/mL, with storage at 2-8°C for 12 months.

Reported linearity falls between 81% and 105% across serum, EDTA plasma and heparin plasma at dilutions of 1:2, 1:4 and 1:8. Recovery ranges are 90-104% (average 97%) for serum, 88-103% (average 96%) for EDTA plasma, and 88-104% (average 94%) for heparin plasma. The protocol involves primary incubation at 37°C for 90 minutes, biotin-labeled detection antibody binding for 60 minutes, HRP-Streptavidin binding for 30 minutes, TMB colour development for 10-20 minutes in the dark, and absorbance reading at 450 nm after addition of stop solution.