Mouse Smad4 (Mothers Against Decapentaplegic Homolog 4) ELISA Kit (MOES01273)

This immunoassay from Assay Genie provides a quantitative tool to measure Mothers Against Decapentaplegic Homolog 4 (Smad4) protein in mouse-derived specimens. Smad4 functions as the common-mediator Smad in canonical TGF-β superfamily signaling, partnering with receptor-activated Smads (Smad1, Smad2, Smad3, Smad5, and Smad8) to translocate into the nucleus and regulate transcription of target genes.

The protein is encoded by a tumor suppressor locus originally identified as DPC4 in pancreatic ductal adenocarcinoma, and reduced Smad4 expression or activity has been documented in colorectal carcinoma, head and neck cancers, and juvenile polyposis syndrome. Researchers studying these signaling pathways use the kit in work on epithelial-mesenchymal transition, fibrotic tissue remodeling, gastrointestinal tract development, and immune cell fate decisions.

Reagents are supplied as a complete kit with pre-coated microplate, standards, detection antibody, and buffers. For research use only. Store unopened kit components refrigerated; protect from light; allow equilibration to room temperature before running the assay. Readout is colorimetric and can be captured on a standard plate reader.