Mouse Smad1 (Mothers Against Decapentaplegic Homolog 1) ELISA Kit (MOES01480)

Smad1 (Mothers Against Decapentaplegic Homolog 1) is a receptor-regulated intracellular mediator of bone morphogenetic protein (BMP) signaling, and quantitative measurement of this protein is central to studies of BMP pathway activity. The Assay Genie Mouse Smad1 ELISA Kit provides a colorimetric immunoassay configured for measuring Smad1 in mouse-derived samples, with reagents supplied for 96 individual assays and read on a standard microplate reader.

BMP ligands binding to type I serine/threonine kinase receptors trigger C-terminal phosphorylation of Smad1, enabling complex formation with the co-Smad Smad4, nuclear accumulation, and transcriptional regulation of osteogenic, chondrogenic, and neurogenic target genes. Researchers apply this kit to osteoblast differentiation studies, skeletal and cartilage development models, embryonic patterning investigations, neural development work, and disease contexts including cancer and pulmonary hypertension where BMP-Smad signaling is dysregulated. The assay accommodates a range of mouse-derived sample matrices. Reconstituted reagents require refrigerated storage to preserve activity, and the kit is intended for research use only, supporting in vitro studies of murine BMP-Smad pathway biology.