Mouse Slit Homolog 1 (Slit1) ELISA Kit

Slit Homolog 1 (Slit1) is a large secreted leucine-rich repeat glycoprotein best characterized as an axon guidance cue that signals through Roundabout (Robo) receptors to direct neuronal migration, axon branching, retinal ganglion pathfinding, and axonal fasciculation during nervous system development. This Assay Genie immunoassay kit enables quantitative measurement of Slit1 protein in mouse specimens for research applications spanning developmental neuroscience, kidney morphogenesis, vascular remodeling, leukocyte chemotaxis, and tumor microenvironment biology, where Slit1 has been studied as a context-dependent modulator of angiogenesis and metastatic behavior.

Supplied as a complete kit in a microplate format supporting 96 individual determinations per plate, the package includes pre-coated wells, capture antibody, detection antibody, reference standards, substrate solution, wash buffer, and other assay reagents. The procedure is validated for use with mouse serum or plasma samples and tissue homogenate specimens, with colorimetric readout on standard laboratory plate readers.

Each kit ships with a detailed protocol outlining reagent preparation, sample handling, standard curve generation, and data analysis recommendations. Store refrigerated at 4 degrees C, protect from light, and avoid repeated freeze-thaw of working reagents to preserve assay performance across the full plate.