Mouse SIGLEC3 (Sialic Acid Binding Ig Like Lectin 3) ELISA Kit (MOES01471)

The Assay Genie Mouse SIGLEC3 ELISA Kit is a research-use-only immunoassay tool that quantifies Sialic Acid Binding Ig Like Lectin 3, also known as CD33, in mouse research specimens. SIGLEC3 belongs to the sialic acid-binding immunoglobulin-like lectin family and is expressed predominantly on myeloid lineage cells, where it delivers inhibitory signals through immunoreceptor tyrosine-based inhibitory motifs. Investigators in immunology, hematology, and myeloid leukemia research use this reagent to explore SIGLEC3 expression in murine disease models and to study innate immune regulation.

Each Mouse SIGLEC3 ELISA Kit from Assay Genie is supplied as a complete microplate-format package with reagents sufficient for 96 assays. The procedure uses a pre-coated microplate, biotinylated detection antibody, streptavidin-HRP conjugate, and a chromogenic substrate that produces a colorimetric signal readable on a standard microplate reader. Mouse serum and tissue culture supernatant samples are loaded directly into wells, and a standard curve generated from the included calibrators is used to interpolate unknown SIGLEC3 concentrations across the assay dynamic range.

For reliable performance, equilibrate all reagents to room temperature before initiating the protocol and store the unopened kit under refrigerated conditions. Protect the microplate strips from moisture by returning unused wells to the foil pouch with desiccant. Aliquot the standard stock after reconstitution and minimize freeze-thaw cycles to preserve reagent activity over the shelf life of the product. This Mouse SIGLEC3 ELISA Kit is intended for in vitro research use only and is not approved for diagnostic procedures.