Mouse SH3YL1 (SH3 domain-containing YSC84-like protein 1) ELISA Kit (MOES01825)

SH3YL1, a cytoplasmic scaffold protein that binds phosphoinositides through its SYLF domain and partners with actin regulators during membrane remodeling and lamellipodia formation, can be quantified in mouse-derived research samples using this immunoassay from Assay Genie. Investigators working on cytoskeletal dynamics, receptor endocytosis, neuronal morphogenesis, or the reported involvement of SH3YL1 in hepatocellular carcinoma and related tumor models use the assay to track endogenous protein concentrations in cell culture and tissue specimens.

The kit ships with components sufficient for 96 individual determinations, including a pre-coated microplate, lyophilized recombinant standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, chromogenic substrate, and stop solution. The protocol uses a quantitative immunoassay workflow with a colorimetric endpoint read on a standard plate reader, typically completing in several hours from sample loading to final absorbance. Users are advised to equilibrate reagents to room temperature before use, aliquot standards to limit freeze-thaw cycles, and store kit components according to the manual to preserve activity across multiple experimental runs.