Mouse sgp130/Soluble Glucoprotein 130 ELISA Kit

From Assay Genie, this Sandwich ELISA (Double Antibody) kit enables accurate and sensitive colorimetric detection of soluble glycoprotein 130 (sGP130) levels in mouse samples. sGP130 is a key protein involved in inflammatory responses and cytokine signaling pathways, where it plays a crucial role in immune system regulation and is implicated in disease states such as autoimmune disorders, infectious diseases, and inflammatory conditions. The kit is intended for research use in mouse models investigating cytokine signaling and inflammatory responses.

Key performance specifications include a sensitivity of 46.875 pg/mL and a working range of 78.125–5000 pg/mL. The assay is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The workflow involves incubating standards, samples and controls on the pre-coated plate, followed by biotin-labeled detection antibody and HRP-Streptavidin binding, TMB substrate development, and absorbance reading at 450 nm. Kit components are stored at 2–8°C for 12 months.

Reported linearity at 1:2, 1:4 and 1:8 dilutions is 92–100%, 88–104% and 89–105% for serum; 86–99%, 82–90% and 87–100% for EDTA plasma; and 84–100%, 87–101% and 80–104% for heparin plasma (n=5 per matrix). Recovery averages are 94% in serum, 97% in EDTA plasma and 95% in heparin plasma, with ranges of 85–100%, 89–100% and 88–104% respectively (n=5 per matrix).