Mouse Serum amyloid A-3 protein (Saa3) ELISA Kit

From Assay Genie, this Sandwich ELISA is intended for the quantitative measurement of Serum amyloid A-3 protein (SAA3) in mouse samples. SAA3 is described as a major acute-phase reactant and an apolipoprotein of the HDL complex, and is relevant to research in infection, inflammation, and autoimmune disorders. The analyte is a secreted extracellular protein with chemoattractant activity and involvement in I-kappaB phosphorylation.

Compatible sample types include serum, plasma, saliva, urine, cell culture supernatant, tissue samples and other related supernatants, and the assay is suited to routine laboratory and HTS microplate formats. Reported performance characteristics include a detection range of 78-5000pg/mL, a sensitivity of 36pg/mL, an Intra-Assay CV of 6.3%, and an Inter-Assay CV of 7.6%. Specificity is for natural and recombinant mouse Serum amyloid A-3 protein (UniProt P04918; observed MW 14kDa, calculated MW 13kDa).

The sandwich ELISA workflow uses a pre-coated microplate, biotinylated detection antibody, and Avidin-HRP conjugate, with signal read at 450 nm ± 2 nm and analyte concentration determined by reference to a standard curve. For research use only.