Mouse Serpina3n ELISA Kit

Manufactured by Assay Genie, this sandwich ELISA kit is designed for the quantitative measurement of Serpina3n — also known as alpha-1-antichymotrypsin — in mouse samples. The double-antibody assay uses colorimetric detection and is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid sample types.

Reagents are stored at 2-8°C for up to 12 months. Linearity has been demonstrated across serum, EDTA plasma, and heparin plasma samples, and recovery ranges from 85-105% depending on the matrix. Synonyms annotated for the target include Spi2, Serine protease inhibitor A3N, and Serpin A3N (UniProt Q91WP6).

Serpina3n is a member of the serpin family implicated in inflammatory responses and tissue remodeling, and has been linked to conditions such as Alzheimer's disease, stroke, and atherosclerosis, supporting its use as a biomarker in immunology research. The protocol includes a 90-minute primary incubation at 37°C, biotin-labeled detection antibody binding, HRP-streptavidin incubation, TMB substrate development, and absorbance measurement at 450 nm.