Mouse Serine/Threonine-Protein Phosphatase 2A 56 kDa Regulatory Subunit Alpha Isoform (PP2Aa) ELISA Kit

The PP2A 56 kDa regulatory subunit alpha isoform is a key scaffolding component of the serine/threonine phosphatase 2A holoenzyme, which governs cell cycle progression, apoptosis, and signal transduction through dephosphorylation of substrates including tau, p53, and components of the MAPK pathway. This quantitative immunoassay kit from Assay Genie enables researchers to measure PP2Aa protein levels in mouse-derived specimens, supporting studies in neuroscience, oncology, and metabolic disease models where phosphatase dysregulation is implicated.

The reagents are supplied as a complete set for use with a standard microplate reader, and the procedure relies on matched antibody recognition of the target analyte immobilized on the plate surface, followed by an enzyme-catalyzed color development step read at the wavelength appropriate for the substrate included in the pack. Pre-coated wells, recombinant standards, detection antibody, wash buffer, and assay buffers are provided to support a full plate workflow, with quantitative curve fitting derived from a freshly prepared standard dilution series.

Mouse samples — including clarified tissue lysates, clarified liquid specimens, and related mouse-derived matrices — serve as validated input types, and performance has been characterized for reproducibility across replicate wells. Recommended handling includes allowing all components to equilibrate to room temperature before use, protecting detection reagents from light, and avoiding repeated freeze-thaw cycles of reconstituted standards. The kit is configured for in vitro research use only and integrates into laboratory workflows targeting phosphatase activity, signaling pathway analysis, and biomarker discovery in mouse models.