Mouse Sepiapterin Reductase (SPR) ELISA Kit

A quantitative immunoassay for measuring sepiapterin reductase (SPR) protein levels in mouse samples, this 96-assay ELISA kit from Assay Genie supports research into tetrahydrobiopterin (BH4) biosynthesis and downstream neurotransmitter pathways. SPR catalyzes the final two reduction steps in BH4 synthesis, converting sepiapterin through 7,8-dihydrobiopterin to the active cofactor, so measuring enzyme levels is relevant to studies of nitric oxide synthase function, aromatic amino acid hydroxylase activity, and dopamine and serotonin production. The assay is validated for tissue homogenates and uses a colorimetric endpoint read on a standard microplate reader. Each kit supplies the pre-coated plate, detection reagents, and buffers required to complete the immunoassay workflow in a research-use-only laboratory protocol. Investigators working with mouse models of SPR deficiency, hyperphenylalaninemia, or other BH4-related metabolic disorders can use this kit to compare enzyme expression across tissues and experimental conditions.