Mouse Semaphorin 7A/SEMA7A ELISA Kit

Assay Genie provides a colorimetric Sandwich ELISA, Double Antibody kit for quantitative measurement of mouse Semaphorin 7A (SEMA7A). The assay is designed for research use, with reactivity specific to mouse samples including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

The kit reports a sensitivity of 0.188 ng/mL and a measuring range of 0.313-20 ng/mL. According to the manufacturer, SEMA7A is a key protein involved in cell signaling and immune responses, contributing to cell migration, axon guidance, and immune cell activation, with dysregulation linked to inflammatory diseases, autoimmune disorders, and cancer. Storage is specified at 2-8°C for 12 months, and the research area is identified as Developmental Biology.

Performance characteristics include recovery data for serum (range 85-101%, average 95%), EDTA plasma (range 95-105%, average 98%), and heparin plasma (range 85-101%, average 95%). Linearity data is provided across dilutions of 1:2, 1:4, and 1:8 for the same sample types. The assay procedure uses incubation at 37°C, with steps including primary incubation (90 minutes), detection antibody binding (60 minutes), HRP-Streptavidin binding (30 minutes), and TMB color development in the dark for 10-20 minutes, with absorbance measured at 450 nm.