Mouse Semaphorin 3F/SEMA3F ELISA Kit

A Sandwich ELISA kit from Assay Genie designed for the quantitative measurement of mouse Semaphorin 3F (SEMA3F) in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The assay uses a double antibody sandwich format with colorimetric detection, offering a sensitivity of 0.188 ng/mL across a detection range of 0.313-20 ng/mL. Kit components should be stored at 2-8°C for 12 months.

SEMA3F is described as a key protein involved in regulating axon guidance and inhibiting angiogenesis, with dysregulation implicated in cancer, autoimmune disorders, and developmental abnormalities. The protocol involves primary incubation at 37°C for 90 minutes, biotin-labeled detection antibody binding for 60 minutes, HRP-Streptavidin binding for 30 minutes, TMB color development for 10–20 minutes in the dark, and absorbance measurement at 450 nm.

Validated linearity spans 80–104% across serum, EDTA plasma, and heparin plasma at 1:2, 1:4, and 1:8 dilutions, with reported average recovery of 98% for serum, 91% for EDTA plasma, and 97% for heparin plasma.