Mouse Semaphorin 3E (SEMA3E) ELISA Kit

Semaphorin 3E (SEMA3E) is a secreted class 3 semaphorin that signals through the plexin-D1 receptor, contributing to axon guidance, vascular patterning during angiogenesis, and remodeling of the tumor microenvironment in mouse models of disease. The Mouse Semaphorin 3E ELISA kit from Assay Genie provides a quantitative immunoassay for measuring SEMA3E concentrations in murine laboratory samples.

Laboratories investigating neuroscience, vascular biology, or tumor biology apply this assay kit to quantify circulating and tissue-resident SEMA3E. The protocol is configured for in vitro research use only and supports quantitative analysis of mouse specimens including tissue homogenates, cell lysates, plasma, and serum. The complete kit ships in a single pack of reagents containing the microplate, lyophilized recombinant standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer concentrate, and substrate solution packed in individual vials.

Before initiating the assay, equilibrate all components to room temperature and store the unopened kit refrigerated between runs. Prepared specimens should be aliquoted into single-use portions and freeze-thaw cycles avoided to preserve analyte integrity. The microplate format with a colorimetric end-point signal is read on standard laboratory absorbance plate readers, allowing the assay to be integrated into common immunology, cell culture, and molecular biology workflows.