Mouse SELL (L-Selectin) ELISA Kit (MOES01145)

This quantitative ELISA kit from Assay Genie measures L-selectin (CD62L) protein levels in mouse research samples. L-selectin is a cell-surface adhesion molecule expressed on leukocytes that mediates lymphocyte homing to peripheral lymph nodes and supports the initial rolling step of neutrophils along vascular endothelium during inflammatory responses. Researchers use this assay to study leukocyte trafficking, immune cell migration, vascular inflammation, and related immunological mechanisms.

The colorimetric microplate-based assay is supplied with capture antibody-coated wells, detection reagent, calibrated protein standards, substrate solution, wash buffer, and a detailed protocol. Following the standard ELISA workflow of incubation, washing, and color development, absorbance is measured to determine sample L-selectin concentrations against the standard curve.

Typical applications include monitoring soluble L-selectin shedding during inflammation, profiling immune responses in murine disease models, and quantifying leukocyte activation markers in transplantation, autoimmunity, sepsis, and allergy research. Compatible sample types include cell culture supernatants, tissue homogenates, and body fluids collected from mouse experimental systems.

Reagents should be stored refrigerated upon arrival and protected from light. Allow all components to equilibrate to room temperature before use, and avoid repeated freeze-thaw cycles of reconstituted standards. For research use only, in vitro laboratory applications.