Mouse Secretory Immunoglobulin A (sIgA) ELISA Kit

Quantitative enzyme-linked immunosorbent assay from Assay Genie for measuring secretory immunoglobulin A concentrations in mouse research specimens. The kit supports investigation of mucosal immunity, including studies of gut-associated lymphoid tissue, respiratory defenses, intestinal microbiota interactions, and mucosal vaccine responses. Secretory IgA serves as a key biomarker for evaluating host protection at epithelial barriers, and quantifying it in mouse models helps researchers characterize immune involvement in colitis, allergy, infection models, and oral or intranasal vaccination protocols.

The colorimetric assay procedure uses a plate-based format with reagents supplied ready for laboratory use. Compatible specimens include serum, EDTA plasma, saliva, intestinal lavage fluid, bronchoalveolar lavage samples, fecal extracts, and clarified tissue homogenates. Standard curves generated from the included calibrators allow interpolation of unknown sample values, and the protocol typically requires a small volume of each specimen added to wells pre-coated with capture reagents specific for the secretory component and the immunoglobulin A heavy chain.

This assay is intended for research use only in academic, pharmaceutical, and biotechnology laboratories investigating mucosal antibody production, IgA class switching, plasma cell function, and transepithelial transport of immunoglobulins. The plate format allows batch processing of experimental and control specimens alongside standard curves, supporting workflows in immunology, microbiology, gastroenterology, and vaccine development programs.