Mouse Secreted Frizzled Related Protein 5 (SFRP5) ELISA Kit

Quantitative measurement of mouse Secreted Frizzled Related Protein 5 (SFRP5) is supported by this solid-phase immunoassay kit from Assay Genie, configured for 96 assays per kit. SFRP5 functions as an endogenous modulator of the Wnt/β-catenin pathway and is predominantly secreted by mature adipocytes, where it has been implicated in metabolic regulation, adipose tissue inflammation, and insulin signaling. Investigators studying obesity-linked metaflammation, type 2 diabetes, hepatic steatosis, and adipocyte biology routinely use this ELISA to quantify circulating SFRP5 concentrations in murine model systems.

The procedure follows a standard quantitative immunoassay workflow read on a microplate reader, making it compatible with existing laboratory pipelines. Each kit is supplied with the reagents required for analysis and a microplate pre-coated with capture antibody specific to mouse SFRP5. Reconstituted standards and diluted samples are added to the wells, followed by detection antibody and substrate development, allowing construction of a standard curve from which SFRP5 levels in unknown samples are interpolated. Storage of unopened kits and reagents should follow the manufacturer's recommendations to preserve antibody binding activity and substrate stability across repeated freeze-thaw cycles.

The kit is intended for research use only and supports both in vitro and in vivo study designs where accurate quantification of mouse SFRP5 is required across multiple specimen types and tissue lysates in basic and translational research settings.