Mouse SDF-1 beta (Stromal Cell Derived Factor 1 Beta) ELISA Kit (MOES01506)

From Assay Genie, this quantitative kit measures murine CXCL12, the beta isoform of stromal cell-derived factor 1 that signals through CXCR4 and ACKR3 to direct hematopoietic stem cell homing, B-cell maturation in marrow, cardiac and neural progenitor migration, and inflammatory cell recruitment after tissue damage. The reagents support 96 individual determinations using a pre-coated microplate strip format, a biotinylated detection antibody, streptavidin-HRP, and a lyophilized recombinant standard calibrated to generate a curve from approximately 15.6 to 1000 pg/mL with a detection threshold near 9.4 pg/mL. Users typically dispense 100 µL of diluted sample per well, incubate, wash, and quantify chemokine concentration from absorbance readings. The assay is applied in studies of bone marrow transplantation, ischemic reperfusion injury, rheumatoid arthritis models, and tumor microenvironment research in mice. Reagents arrive refrigerated and remain stable when stored at 4 °C; reconstituted standard and detection antibody should be aliquoted to limit repeated freeze-thaw cycles.