Mouse sCD163 (Soluble Cluster of Differentiation 163) ELISA Kit (MOFI01441)

Soluble CD163 (sCD163), the shed extracellular portion of the macrophage-specific hemoglobin-haptoglobin scavenger receptor CD163, rises in circulation during systemic inflammation, hemolysis, and macrophage activation. The Mouse sCD163 ELISA Kit from Assay Genie is a quantitative immunoassay built to measure this biomarker in mouse research specimens, supporting studies in sepsis models, acute and chronic liver injury, NAFLD and NASH research, hemophagocytic lymphohistiocytosis, atherosclerosis, and Gaucher disease.

Supplied in a 96 Assay configuration, the kit contains a pre-coated microplate, lyophilized standards, a biotinylated detection antibody, streptavidin-HRP conjugate, chromogenic substrate solution, and assay buffers sufficient for a full standard curve and multiple sample replicates. The substrate-based endpoint is read on a standard microplate reader, allowing laboratories to integrate the workflow with existing plate-reader infrastructure. Researchers typically load small serum-type or plasma-type sample volumes per well following the included protocol, with EDTA-treated plasma commonly used alongside serum preparations. Unused reagents should be refrigerated between uses to preserve antibody and conjugate activity; avoid repeated freeze-thaw cycles of reconstituted standards and detection reagents. For research use only; not intended for diagnostic or clinical applications.