Mouse SCARB1 (Scavenger receptor class B member 1) ELISA kit (MOES00972)

The Mouse SCARB1 ELISA kit from Assay Genie detects endogenous mouse Scavenger Receptor Class B Member 1 (UniProt Q61009; NCBI Gene ID 20778), the multi-pass membrane protein that localizes predominantly to plasma membrane caveolae and binds PDZK1 through its C-terminal region. The protocol uses a 100 µL sample volume per well and typically completes within 3.5 hours of incubation at 37 °C. The microplate is supplied as 8 × 12 removable strips, paired with two vials of lyophilized standard, 20 mL of sample diluent, 10 mL each of assay diluents A and B, 120 µL of detection reagents A and B, 30 mL of wash buffer, 10 mL each of TMB substrate and stop solution, and five plate sealers. Centrifuge liquid samples at 1000 × g for 20 minutes before use; aliquot reconstituted standards into single-use vials and store the microplate, standards, and detection reagents at -20 °C, while wash buffer, substrate, and stop solution remain at 4 °C. Investigators focused on HDL-driven reverse cholesterol transport, adrenal lipid uptake, or hepatic lipid homeostasis in murine models can apply this reagent to atherosclerosis, diabetes, and metabolic syndrome research.