Mouse Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 (Atp2a1) ELISA Kit

A sandwich ELISA from Assay Genie designed for the quantitative measurement of mouse Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 (ATP2A1) in serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids. The assay is reactive with mouse samples and is intended for research use only.

Reported performance characteristics include a detection range of 78-5000pg/mL and a sensitivity of 39.8pg/mL. Intra-assay CV is 6.2% and inter-assay CV is 7.9%. Linearity across dilutions (1:2, 1:4, 1:8, 1:16) is reported for serum (99-111%, 108-120%, 109-118%, 99-109%), EDTA plasma (103-113%, 109-119%, 105-115%, 99-109%) and heparin plasma (97-108%, 86-96%, 86-96%, 95-105%). Recovery averages 86% (80-92%) for serum and 88% (82-94%) for plasma. ATP2A1 is described as a magnesium-dependent enzyme that hydrolyses ATP coupled with translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, contributing to calcium sequestration involved in muscular excitation and contraction.

Synonyms listed include Calcium pump 1, Calcium-transporting ATPase sarcoplasmic reticulum type (fast twitch skeletal muscle isoform), Endoplasmic reticulum class 1/2 Ca(2+) ATPase and SERCA1. The associated UniProt accession is Q8R429 and the research area is Neurosciences. The assay detects natural and recombinant mouse Sarcoplasmic/endoplasmic reticulum calcium ATPase 1.