Mouse S1PR4/ EDG6 ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA kit is developed for the precise measurement of S1PR4 (also known as EDG6) levels in mouse samples. The assay uses a double-antibody sandwich format with colorimetric detection and is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. S1PR4 is described as a key receptor involved in the regulation of immune responses and cell migration, with relevance to inflammatory diseases, autoimmune conditions, and cancer progression.

The kit offers a sensitivity of 46.875 pg/mL and a measurable range of 78.125-5000 pg/mL. Reported linearity spans 80% to 105% across serum, EDTA plasma, and heparin plasma at 1:2, 1:4, and 1:8 dilutions, while recovery averages are documented as 97% for serum, 96% for EDTA plasma, and 99% for heparin plasma. Storage is specified at 2-8°C for 12 months. The assay procedure includes reagent and plate preparation, a 37°C primary incubation, biotin-labeled detection antibody binding, HRP-streptavidin (SABC) binding, TMB substrate color development, and absorbance measurement at 450 nm after addition of stop solution.